Mechanism of Urea Conduction through H. Pylori UreI
نویسندگان
چکیده
منابع مشابه
A H+-gated urea channel: the link between Helicobacter pylori urease and gastric colonization.
Acidic media trigger cytoplasmic urease activity of the unique human gastric pathogen Helicobacter pylori. Deletion of ureI prevents this activation of cytoplasmic urease that is essential for bacterial acid resistance. UreI is an inner membrane protein with six transmembrane segments as shown by in vitro transcription/translation and membrane separation. Expression of UreI in Xenopus oocytes r...
متن کاملUrea Breath Test (UBT) shows high prevalence of Helico bacter pylori (H. pylori) infection in patients with dyspepsia in southern Iran.
Introduction: Helicobacter pylori (H. pylori) is suggested to play a role in dyspepsia. The aim of this study was to assess the prevalence of H. pylori, in dyspeptic patients. Methods: This cross-sectional study was conducted on 850 patients with dyspepsia in Iran (2006-2007). The patients were selected using convenience sampling method. Data was collected using ROME III criteria questionnaire...
متن کاملLocal pH elevation mediated by the intrabacterial urease of Helicobacter pylori cocultured with gastric cells.
Helicobacter pylori resists gastric acidity by modulating the proton-gated urea channel UreI, allowing for pH(out)-dependent regulation of urea access to intrabacterial urease. We employed pH- and Ca(2+)-sensitive fluorescent dyes and confocal microscopy to determine the location, rate, and magnitude of pH changes in an H. pylori-AGS cell coculture model, comparing wild-type bacteria with nonpo...
متن کاملExpression of the Helicobacter pylori ureI gene is required for acidic pH activation of cytoplasmic urease.
ureI encodes an integral cytoplasmic membrane protein. It is present in the urease gene cluster of Helicobacter pylori and is essential for infection and acid survival, but its role is unknown. To determine the function of UreI protein, we produced H. pylori ureI deletion mutants and measured the pH dependence of urease activity of intact and lysed bacteria and the effect of urea on the membran...
متن کاملThe Helicobacter pylori UreI protein is not involved in urease activity but is essential for bacterial survival in vivo.
We produced defined isogenic Helicobacter pylori ureI mutants to investigate the function of UreI, the product of one of the genes of the urease cluster. The insertion of a cat cassette had a strong polar effect on the expression of the downstream urease genes, resulting in very weak urease activity. Urease activity, measured in vitro, was normal in a strain in which ureI was almost completely ...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2016
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2015.11.2917